Navratilova - two defeats on Saturday (Getty Images)

Martina Navratilova must wait another year and until the eve of her 50th birthday if she is to become the outright record-holder of 21 Wimbledon crowns.

The nine-times singles champion who has won close to US$22million in prize money, lost hope of moving ahead of American star Billie-Jean King's 20 victories with two doubles defeats in one day in the semi-finals of the women's and quarter-finals of the mixed doubles.

She and German partner Anna-Lena Groenefeld lost 6-4 6-4 to Amelie Mauresmo and Svetlana Kuznetsova before she and Mike Bryan went down in a two-hour 14-minute thriller against Zimbabwe's Kevin Ullyett and South Africa's Liezl Huber.

They won the first set in good style and in the decider Martina served for the match but dropped her delivery before the African pair triumphed 3-6 6-4 9-7.

Navratilova, who still has a career-total of 167 singles and 174 doubles crowns, was seeded eight in the women's event with Groenefeld, but Mauresmo and Kuznetsova, a new combination and unseeded, beat them in an hour and eight minutes earlier.